Waa maxay sababta Polyacrylamide loo isticmaalo borotiinka electrophoresis

Dhinaca sayniska casriga ah, borotiinka elektrophoresis wuxuu u taagan yahay farsamo aasaasi ah oo lagu falanqeeyo laguna garto borotiinnada.Udub-dhexaadka habkani waa beenPolyacrylamide, xarun isku dhafan oo u adeegta sida laf-dhabarka matriks jel ee loo isticmaalo hababka electrophoresis jel.Guryaha gaarka ah ee Polyacrylamide waxay ka dhigaan qalab lagama maarmaan u ah cilmi-baarayaasha iyo saynisyahannada doonaya inay soo bandhigaan kakanaanta borotiinnada iyo isdhexgalka.

Polyacrylamide, oo inta badan loo yaqaan PAM, waa polymer synthetic oo laga sameeyay monomers acrylamide.Kala duwanaanshaheeda cajiibka ah waxaa loo aaneynayaa awooda ay u leedahay sameynta silsilado dhaadheer, taasoo keentay walax jel oo kale ah oo qaadi karta qiyaaso kala duwan oo unugyo ah.Hantidani waxay ka dhigaysa polyacrylamide musharax ku habboon abuurista matries dalool leh oo loo isticmaalo borotiinka electrophoresis.

Protein electrophoresis waa farsamo kala saarta borotiinnada iyadoo loo eegayo kharashkooda iyo cabbirkooda.Iyada oo la gelinayo muunadda borotiinka goob koronto oo ku dhex jirta matrix gel polyacrylamide, borotiinadu waxay u guuraan jeelka heerar kala duwan, taasoo keenta kooxo kala duwan oo la lafaguro lana qiyaasi karo.Kala soocidaani waxay bixisaa fikrado qiimo leh oo ku saabsan nadiifnimada borotiinka, go'aaminta miisaanka molecular, iyo joogitaanka isoforms.

Doorka Polyacrylamide ee Protein Electrophoresis

Doorashada polyacrylamide ee borotiinka electrophoresis waxay ku saleysan tahay dabeecadeeda la hagaajin karo.Saynis yahanadu waxay hagaajin karaan fiirsashada matrix gel si ay u habeeyaan borotiinada cabbirrada kala duwan.Xoojinta sare waxay abuurtaa matries adag oo ku habboon in lagu xalliyo borotiinnada yaryar, halka uruurinta hoose loo isticmaalo borotiinno waaweyn.La qabsigani waxa uu hubinayaa in cilmi-baarayaashu ay u habayn karaan tijaabadooda si ay u gaadhaan kala saarid iyo falanqayn wanaagsan.

Polyacrylamide sida aFlocculant

Utility Polyacrylamide wuxuu ku fidsan yahay in ka badan doorka uu ku leeyahay jel electrophoresis.Waxa kale oo ay u heshaa codsiyada sida qulqulo badan oo warshado kala duwan ah, sida daaweynta biyaha iyo maaraynta biyaha wasakhda ah.Flocculant ahaan, polyacrylamide waxay caawisaa isku-darka walxaha laalladay ee dareeraha ah, taasoo fududaynaysa ka saaristooda.Dabeecaddani waxay muujinaysaa awoodaha kala duwan ee xarunta iyo saamaynta balaadhan ee sayniska iyo warshadaha.

Horumarka Electrophoresis-ku-saleysan Polyacrylamide

Sannadihii ugu dambeeyay waxay goob joog u ahaayeen horumarro isdaba joog ah oo ku saabsan farsamooyinka elektrophoresis-ku-saleysan ee polyacrylamide.PAGE Native, SDS-PAGE, iyo laba-cabbir jel electrophoresis waa kaliya dhowr tusaale oo ah sida laqabsiga polyacrylamide uu u suurtageliyay horumarinta habab gaar ah oo lagu falanqeeyo qaabdhismeedka borotiinka, isbeddelada tarjumaada ka dib, iyo isdhexgalka.Farsamooyinkani waa kuwo qiimo weyn u leh cilmi-baarista borotokoolka iyo dadaalka helitaanka daroogada.

Dhinaca falanqaynta borotiinka, polyacrylamide waxay u soo baxdaa saaxiib adag, taas oo awood u siinaysa cilmi-baarayaasha inay dhexgalaan adduunka adag ee borotiinka.Doorkeeda aasaasiga ah ee matrices jel ee nidaamyada elektiroonigga ah lama soo koobi karo.Laga soo bilaabo hababka cudurka ee furfuran ilaa horumarinta daawaynta cusub, electrophoresis ku salaysan polyacrylamide waxay sii wadaa inay qaabayso horumarka sayniska.Marka ay tignoolajiyadu horumarto, yaababkan synthetic waxa ay u badan tahay in uu kobcayo, iyada oo sii kobcinaysa fahamkayaga borotiinnada iyo hawlahooda faraha badan.