Waa maxay sababta Polyacrylamide loo isticmaalo electrophoresis-ka borotiinka

Dhinaca sayniska casriga ah, electrophoresis-ka borotiinka ayaa ah farsamo asaasi ah oo lagu falanqeeyo laguna garto borotiinnada.Polyacrylamide, isku-darka kala duwan oo u adeega laf-dhabarta matrices-ka jelka ee loo isticmaalo nidaamyada electrophoresis-ka jelka. Astaamaha gaarka ah ee Polyacrylamide waxay ka dhigayaan qalab lama huraan u ah cilmi-baarayaasha iyo saynisyahannada doonaya inay xalliyaan dhibaatooyinka borotiinnada iyo is-dhexgalka.

Polyacrylamide, oo inta badan loo yaqaan PAM, waa polymer macmal ah oo laga sameeyay monomers-ka acrylamide. Kala duwanaanshaheeda cajiibka ah waxaa loo aaneeyaa awoodda ay u leedahay inay samayso silsilado dhaadheer, taasoo keenta walax u eg jel oo qaadi karta cabbirro kala duwan oo molecules ah. Sifadan waxay ka dhigaysaa polyacrylamide musharax ku habboon abuurista matrices daloolo leh oo loo isticmaalo electrophoresis-ka borotiinka.

Elektroforesis-ka borotiinka waa farsamo kala soocda borotiinada iyadoo lagu saleynayo dallacooda iyo cabbirkooda. Iyadoo muunad borotiin ah lagu shubayo goob koronto oo ku jirta matrix jel polyacrylamide ah, borotiinadu waxay u gudbaan jelka iyagoo ku jira heerar kala duwan, taasoo keenta xargo kala duwan oo la falanqeyn karo oo la cabbiri karo. Kala soociddani waxay bixisaa aragtiyo qiimo leh oo ku saabsan daahirnimada borotiinka, go'aaminta miisaanka molecular, iyo joogitaanka isoforms.

Doorka Polyacrylamide ee Borotiinka Electrophoresis

Doorashada polyacrylamide ee loogu talagalay electrophoresis-ka borotiinka waxay ku saleysan tahay dabeecadeeda la hagaajin karo. Saynisyahannadu waxay hagaajin karaan isku-darka matrix-ka jel si ay ula jaanqaadaan borotiinnada cabbirrada kala duwan. Isu-ururinta sare waxay abuurtaa matrices adag oo ku habboon xallinta borotiinnada yaryar, halka isku-ururinta hoose loo isticmaalo borotiinnada waaweyn. La qabsigan wuxuu hubinayaa in cilmi-baarayaashu ay u habeyn karaan tijaabooyinkooda si ay u gaaraan kala-soocid iyo falanqayn ugu habboon.

Polyacrylamide oo loo isticmaalo sidii xalFlocculant

Faa'iidada Polyacrylamide waxay ka baxsan tahay doorka ay ku leedahay electrophoresis-ka jel. Waxay sidoo kale u heshaa codsiyo ah flocculant warshado kala duwan, sida daaweynta biyaha iyo maaraynta biyaha wasakhda ah. Iyada oo ah flocculant, polyacrylamide waxay ka caawisaa isu-geynta walxaha la hakiyey ee dareeraha, taasoo fududaynaysa ka saarista. Astaantan waxay iftiiminaysaa awoodaha kala duwan ee isku-dhafka ah iyo saameynta ballaaran ee sayniska iyo warshadaha.

Horumarka Electrophoresis-ka ku salaysan Polyacrylamide

Sannadihii ugu dambeeyay waxaa la arkay horumar joogto ah oo ku yimid farsamooyinka electrophoresis-ka ku salaysan polyacrylamide. PAGE-ga Native, SDS-PAGE, iyo electrophoresis-ka jel-ka laba-geesoodka ah ayaa ah dhowr tusaale oo ku saabsan sida la qabsiga polyacrylamide uu u suurtageliyay horumarinta habab gaar ah oo lagu falanqeeyo qaab-dhismeedka borotiinka, wax-ka-beddelka turjumaadda kadib, iyo isdhexgalka. Farsamooyinkani waa kuwo qiimo leh oo ku jira cilmi-baarista proteomics iyo dadaallada ogaanshaha daroogada.

Marka laga hadlayo falanqaynta borotiinka, polyacrylamide waxay u soo baxdaa sidii saaxiib adag, taasoo u suurtagelinaysa cilmi-baarayaasha inay baaraan adduunka adag ee borotiinnada. Doorkeeda aasaaska matrices-ka jel ee nidaamyada electrophoresis lama soo koobi karo. Laga bilaabo furfuridda hababka cudurrada ilaa horumarinta daaweynta cusub, electrophoresis-ka ku salaysan polyacrylamide wuxuu sii wadaa inuu qaabeeyo horumarka sayniska. Marka tignoolajiyadu horumarto, yaabkan macmalka ah wuxuu u badan yahay inuu kobco, taasoo sii xoojinaysa fahamkeenna borotiinnada iyo shaqooyinkooda aan la tirin karin.