Boqortooyada dhabta ah ee sayniska casriga ah, elecilka proteinesis-ka ee borotophoresis wuxuu u taagan yahay farsamada aasaasiga ah ee falanqaynta iyo lagu garto borotiinnada. Wadnaha qaabkan ayaa been ahPolyacchalamide, xarun isku mid ah oo u adeegta laf dhabar u ah matajooyinka gel ee loo adeegsaday nidaamyada elektrophoresis gel. Sifooyinka gaarka ah ee cudurka 'polyacchalamin' ee loo yaqaan 'polyacchalamides' ee 'polyacchalamides' ee u qalma ee loogu talagalay cilmi-baarayaasha iyo saynisyahannada raadinaya inay soo saaraan kakanaanta borotiinnada iyo is-dhexgalka.
Polyacchalamide, oo badanaa loo yaqaan pam, waa polymer isku-darka polytheres oo laga sameeyay monomers-ka acrylamidel. Awoodda ay waxtarka leh ayaa loo aaneeyey awooda ay u leedahay in la sameeyo silsilado dhaadheer, taasoo dhalisay walax u eg gel-sida qaadi kara cabirro kala duwan oo moleekules. Hantidan ayaa ka dhigaysa polyacchium-ga proyacryluside ah oo ku habboon abuurista matrous jilicsan oo loo adeegsaday proteophoresis.
Borotiinka elektaroonigga ah waa farsamo kala saarta borotiinnada ku saleysan eedeyntooda iyo cabirkooda. Adigoo u horseedaya muunad borotiin ah oo ku jira garoonka korantada ee ku dhex jira pelyacchikelamides gel matrix, borotiinno u guuray iyada oo loo marayo jel sicir kala duwan, taasoo keentay in xargaha kala duwan ee la falanqi karo oo la eedeeyo. Kala go'itaankaani wuxuu bixiyaa aragtiyo qiimo leh oo lagu sameeyo daahirsanaanta borotiinka, goynta miisaanka molical, iyo jiritaanka himorms.
Doorka cudurka polyacrilamide ee protein electrophoresis
Xulashada cudurka 'polyacchachiemin' ee borotiinka elektiroonigga ah ee protein electrophoresis-ka ayaa ku xidhmay dabeecadiisa aan la isticmaali karin. Saynisyahannadu waxay hagaajin karaan uruurinta matrix-ka Gel si loo habeeyo borotiinno cabbirro kaladuwan ah. Uruurinta Sare ee Sare u abuuraan matricyo adag oo ku habboon xallinta borotiinka yaryar, halka ururinta hoose loo isticmaalo borotiinka waaweyn. Laqabsashadan waxay xaqiijineysaa in cilmi-baarayaashu ku habeyn karaan tijaabayaashooda si loo gaaro kala-goynta ugu habboon iyo falanqaynta.
Polyacchium sida aDicculant
Adeegga cudurka polyacchalamide wuxuu ku fidsan yahay ka baxsan doorkeeda ee Gel Encerophoresis. Waxay sidoo kale u aragtaa codsiyo sida Flocculant ee warshado kala duwan, sida daaweynta biyaha iyo maaraynta biyaha wasakhda ah. Sida Flocculant, Qalabka cudurka 'polyacrylamide' ee isku darka isugeynta walxaha kacsan ee cabitaannada, fududeynta ka saaritaankooda. Astaamahani wuxuu iftiiminayaa awoodda kaladuwan ee xarunta iyo saameynta ballaaran ee sayniska iyo warshadaha.
Kordhinta cudurka 'polyacchalamide-ku-saleysan prolacchiesis'
Sanadihii la soo dhaafay waxay arkeen horumar sii socota oo horumar ka sameysan farsamooyinka ku saleysan cudurka polyacchalamide ee ku saleysan cudurka 'electrophoresis ku saleysan cudurka' proyacchiesis '. Bogga hooyo, bogga-SD--da, iyo Ereen-ka-enchophoresis laba-geesood ah ayaa ah tusaalooyin dhawr ah oo ka mid ah qaab-dhismeedka loo yaqaan 'polacylamide' ee loo habeeyey horumarinta hababka takhasuska leh ee lagu falanqayo qaabdhismeedka borotiinka, wax ka beddelka tarjumaadda kadib, iyo is-dhexgalka. Farsamooyinkan ayaa muhiim u ah cilmi baarista cilmi-baarista iyo dadaallada daahfurka daroogada.
On cudurka falanqaynta borotiinka, polyacriamidel waxay u soo baxdaa sidii wehelka stalwart, oo u oggolaanaya cilmi-baarayaal si ay ugu galaan adduunka xasaasiyadda ee borotiinnada. Doorkeedu yahay aasaaska mataanaha Gel ee nidaamyada elektrophoresis-ka ma dhaafi karo. Laga soo bilaabo ka soo baxa hababka cudurka si loo horumariyo daweynta sheeko-sheeko, cudurka 'polyacryriesis-ku ku saleysan elektrophoresis-ka ku saleysan polrophoresis wuxuu sii wadaa qaabaynta horumarka sayniska. Maaddaama ay tahay horumarinta tikniyoolajiyadda, Marjafkan Isku-darka ah waxay u egtahay inay kobciso, taasoo sii xoojineysa fahamkayaga borotiinnada iyo howlahooda Myriad.
Waqtiga Post: Agoosto-21-2023